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A16, A 16, A.16 or A-16 may refer to:
- A16 road, in several countries
- ATC code A16, Other alimentary tract and metabolism products, a subgroup of the Anatomical Therapeutic Chemical Classification System
- British NVC community A16 (Callitriche stagnalis community), a British Isles plant community
It may also refer to:
- A16, a restaurant in the San Francisco Bay Area
- Subfamily A16, a rhodopsin-like receptors subfamily
- One of the Encyclopaedia of Chess Openings codes for the English Opening in chess
- Washington A16, 2000 Protests in Washington, D.C. against the International Monetary Fund and the World Bank on April 16 of 2000 and 2005, both known as "A16"