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Dr. No may refer to:
- Dr. No (novel), a 1958 James Bond novel by Ian Fleming
- Dr. No (film), a 1962 film based on the novel
- Julius No, the title character in the Fleming novel and film adaptation
- Dr. No (serial killer), a nickname given to serial killer Samuel Legg III who was theorized to be active in Ohio between 1981 and 1990
- "Dr. No", The Hogan Family season 2, episode 6 (1986)
- "Dr. No" (song), a song by Systems in Blue
- Dr. No?, a 1991 documentary by Jacob Young about William Luther Pierce
- Dr. No: A Novel, a 2022 book by Percival Everett