Harley Quinn is a character appearing in comic books published by DC Comics.
Harley Quinn may also refer to:
- Harley Quin, a character appearing in works by Agatha Christie
- Harley Quinn (comic book), an ongoing series published by DC Comics featuring the titular character as the protagonist
- Harley Quinn (DC Extended Universe)
- Harley Quinn, an alternate title for Birds of Prey (2020 film), a 2020 American film based on the DC Comics team the Birds of Prey
- Harley Quinn (pornographic actor)
- Harley Quinn (TV series), an American animated television series based on the DC Comics character
- Harley Quinn Smith, daughter of Kevin Smith
- "Harley Quinn" (song), by Fuerza Regida and Marshmello, 2023