Hi-C may refer to:
- Hi-C, a fruit-flavored beverage brand owned by The Coca-Cola Company
- Hi-C (rapper), a Compton, California, rapper
- Hi-C (genomic analysis technique), a variant of the Chromosome conformation capture technique, used to study the conformation of an entire genome
- High Resolution Coronal Imager, a space telescope designed to take images of the Sun's atmosphere
- The C (musical note) above the top of the staff in treble clef
- Hi-C, a Nashville, Tennessee, rapper