Back الإنزيم المثبت Arabic Enzima inmovilizada Spanish تثبیت آنزیم Persian קיבוע אנזימים HE Enzim terimobilisasi ID Immobilized enzyme SIMPLE 固定化酶 Chinese
An immobilized enzyme is an enzyme, with restricted mobility, attached to an inert, insoluble material—such as calcium alginate (produced by reacting a mixture of sodium alginate solution and enzyme solution with calcium chloride). This can provide increased resistance to changes in conditions such as pH or temperature. It also lets enzymes be held in place throughout the reaction, following which they are easily separated from the products and may be used again - a far more efficient process and so is widely used in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization.[1][2] Immobilized enzymes are easily to be handled, simply separated from their products, and can be reused.[3]
Enzymes are bio-catalysts which play an essential role in the enhancement of chemical reactions in cells without being persistently modified, wasted, nor resulting in the loss of equilibrium of chemical reactions. Although the characteristics of enzymes are extremely unique, their utility in the industry is limited due to the lack of re-usability, stability, and high-cost of production.[4]
- ^ Zaushitsyna O, Berillo D, Kirsebom H, Mattiasson B (2013). "Cryostructured and Crosslinked Viable Cells Forming Monoliths Suitable for Bioreactor Applications". Topics in Catalysis. 57 (5): 339–348. doi:10.1007/s11244-013-0189-9. S2CID 94773366.
- ^ Börner RA, Zaushitsyna O, Berillo D, Scaccia N, Mattiasson B, Kirsebom H (2014). "Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production". Process Biochemistry. 49: 10–18. doi:10.1016/j.procbio.2013.09.027.
- ^ Fessner WD (1999). Biocatalysis : from discovery to application. Berlin: Springer. ISBN 3-540-64942-5. OCLC 40551838.
- ^ Zhang DH, Yuwen LX, Peng LJ (2013). "Parameters Affecting the Performance of Immobilized Enzyme". Journal of Chemistry. 2013: 1–7. doi:10.1155/2013/946248. ISSN 2090-9063.