Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry).[1][2] In this approach, antibodies are conjugated with isotopically pure elements, and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argon plasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in flow cytometry by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra.[3]
- ^ Dmitry Bandura; Vladimir Baranov; Olga Ornatsky; Scott D. Tanner; Kinach R; Lou X; Pavlov S; Vorobiev S; Dick JE; Antonov A (2009). "Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry". Analytical Chemistry. 81 (16): 6813–6822. doi:10.1021/ac901049w. PMID 19601617.
- ^ Di Palma, Serena; Bodenmiller, Bernd (2015). "Unraveling cell populations in tumors by single-cell mass cytometry". Current Opinion in Biotechnology. 31: 122–129. doi:10.1016/j.copbio.2014.07.004. ISSN 0958-1669. PMID 25123841.
- ^ Cite error: The named reference
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