Mota'ain, also spelled Motain, Mota'in or Mota Ain, is a hamlet in the Silawan village (desa), East Tasifeto district (kecamatan), Belu Regency, East Nusa Tenggara, Indonesia.
A major border crossing checkpoint with customs, immigration and quarantine services between Indonesia and East Timor, called the Mota'ain Border Crossing Checkpoint, is located in the village. The corresponding checkpoint on the East Timor side is Batugade.
Mota'ain is on the main road between Kupang, 290 km to the southwest, and Dili which is 113 km to the east. The nearest major city is Atambua, the capital of Belu Regency. The port of Atapupu is located 5 km to the west.