Recombinant antibodies are antibody fragments produced by using recombinant antibody coding genes.[1] They mostly consist of a heavy and light chain of the variable region of immunoglobulin. Recombinant antibodies have many advantages in both medical and research applications, which make them a popular subject of exploration and new production against specific targets. The most commonly used form is the single chain variable fragment (scFv), which has shown the most promising traits exploitable in human medicine and research.[2] In contrast to monoclonal antibodies produced by hybridoma technology, which may lose the capacity to produce the desired antibody over time or the antibody may undergo unwanted changes, which affect its functionality, recombinant antibodies produced in phage display maintain high standard of specificity and low immunogenicity.[3][4]
- ^ Creative Biolabs (2017-04-28), Introduction of Recombinant Antibody, retrieved 2017-08-18
- ^ Ahmad, Zuhaida Asra; Yeap, Swee Keong; Ali, Abdul Manaf; Ho, Wan Yong; Alitheen, Noorjahan Banu Mohamed; Hamid, Muhajir (2012). "scFv Antibody: Principles and Clinical Application". Clinical and Developmental Immunology. 2012: 980250. doi:10.1155/2012/980250. ISSN 1740-2522. PMC 3312285. PMID 22474489.
- ^ Kunert R, Reinhart D (April 2016). "Advances in recombinant antibody manufacturing". Appl. Microbiol. Biotechnol. 100 (8): 3451–61. doi:10.1007/s00253-016-7388-9. ISSN 0175-7598. PMC 4803805. PMID 26936774.
- ^ Miltenyi Biotec (2017-03-22), Webinar: Recombinant Antibodies for Improved Flow Cytometry, retrieved 2017-08-20