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Sant'Ambrogio may refer to the following entities in Italy:
- Basilica of Sant'Ambrogio, a church in the Milan
- Sant'Ambrogio, Florence, a Roman Catholic church in Florence
- Sant'Ambrogio di Torino, a municipality in the Turin
- Sant'Ambrogio di Valpolicella, a municipality in Verona, Veneto
- Sant'Ambrogio sul Garigliano, a municipality in Frosinone, Lazio