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Subculture (biology)

"Passaging" redirects here. For the artificial selection method, see Serial passage.
A line of twenty T175 cell culture flasks filled with approximately 40 mL of red cell culture medium
Line of T175 cell culture flasks containing red cell culture medium

In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture.[1]

  1. ^ "Cell Culture Basics" (PDF). Invitrogen. pp. 26–27. Retrieved 20 June 2019.
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