T7 DNA polymerase

DNA-directed DNA polymerase
Figure 1. Crystal structure of T7 DNA replication complex. Rendered from PDB 1T7P.
Identifiers
Organism	Enterobacteria phage T7
Symbol	5
CAS number	9012-90-2
UniProt	P00581
Other data
EC number	2.7.7.7
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T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin,^[1] in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme.^[2] It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase.

This polymerase has various applications in site-directed mutagenesis^[3] as well as a high-fidelity enzyme suitable for PCR.^[4] It has also served as the precursor to Sequenase,^[5] an engineered-enzyme optimized for DNA sequencing.^[6]