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Ntlhoi Motsamai | |
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| Speaker of the National Assembly of Lesotho | |
| In office 10 March 2015 – 3 June 2017 | |
| Preceded by | Sephiri Motanyane |
| Succeeded by | Sephiri Motanyane |
| In office 1999–2012 | |
| Preceded by | John Teboho Kolane |
| Succeeded by | Sephiri Motanyane |
| Personal details | |
| Born | 1963 (age 61–62) Mohale's Hoek District, Lesotho |
| Political party | LCD (to 2012) Democratic Congress (from 2012) |
Ntlhoi Motsamai (born 1963) is a Lesotho politician who served as the first female Speaker of the National Assembly from 1999 to 2012. She was elected again from March 2015 to June 2017. Motsamai worked as a teacher before entering politics.